A hybrid FIA/HPLC system incorporating monolithic column chromatography

Adcock, J, Francis, P, Agg, K, Marshall, G and Barnett, N 2007, 'A hybrid FIA/HPLC system incorporating monolithic column chromatography', Analytica Chimica Acta, vol. 600, no. 12SPEC.ISS., pp. 136-141.

Document type: Journal Article
Collection: Journal Articles

Title A hybrid FIA/HPLC system incorporating monolithic column chromatography
Author(s) Adcock, J
Francis, P
Agg, K
Marshall, G
Barnett, N
Year 2007
Journal name Analytica Chimica Acta
Volume number 600
Issue number 12SPEC.ISS.
Start page 136
End page 141
Total pages 6
Publisher Elsevier BV
Abstract We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2'-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.
Subject Separation Science
Keyword(s) Flow injection analysis
Gradient elution
Monolithic column chromatography
Chemiluminescence detection
Neurotransmitter metabolites
DOI - identifier 10.1016/j.aca.2007.03.048
Copyright notice © 2007 Elsevier B.V. All rights reserved.
ISSN 0003-2670
Version Filter Type
Citation counts: Scopus Citation Count Cited 38 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 199 Abstract Views  -  Detailed Statistics
Created: Wed, 17 Nov 2010, 16:09:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us