A pharmacogenetic study of pregnane X receptor (NR112) in Han Chinese

Wang, X, Li, J, Su, Q, Deng, X, Lu, Y, Chen, J, Zhang, J, Zhao, L, Zhong, Z, Chan, E, Chen, X, Chowbay, B, Xue, C, Huang, M and Zhou, S 2007, 'A pharmacogenetic study of pregnane X receptor (NR112) in Han Chinese', Current Drug Metabolism, vol. 8, pp. 778-786.


Document type: Journal Article
Collection: Journal Articles

Title A pharmacogenetic study of pregnane X receptor (NR112) in Han Chinese
Author(s) Wang, X
Li, J
Su, Q
Deng, X
Lu, Y
Chen, J
Zhang, J
Zhao, L
Zhong, Z
Chan, E
Chen, X
Chowbay, B
Xue, C
Huang, M
Zhou, S
Year 2007
Journal name Current Drug Metabolism
Volume number 8
Start page 778
End page 786
Total pages 9
Publisher Bentham Science
Abstract The pregnane X receptor (PXR/NR1I2) gene is a critical transcriptional regulator of a number of important drug metabolizing enzymes and transporters. This study was undertaken to determine the frequencies of single nucleotide polymorphisms (SNPs) and haplotypes and to detect yet unknown SNPs in the NR1I2 gene in 210 unrelated healthy Han Chinese in comparison with other ethnic groups. We also characterized the functional impact of two SNPs, -24622A>T in the 5'-untranslated region and -24446C>A in exon 1 of NR1I2, by constructing three recombinants and monitoring promoter activity using the dual luciferase reporter gene assay. Genomic DNA was isolated from peripheral leukocytes and subjected to polymerase chain reaction (PCR) amplification, followed by direct DNA sequencing. Sixteen SNPs in NR1I2 with frequencies of 0.3-90.3% were found in Han Chinese, two of which (-25439A>G in the 5'-untranslated region and 7637C>T in intron 5) are previously unknown.
Subject Basic Pharmacology
Keyword(s) Pregnane X Receptor (NR112)
Chinese
Haplotype
ethnicity
DOI - identifier 10.2174/138920007782798199
ISSN 1389-2002
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